The effects of dietary administration of non alkohalic beer concentrate on expression of genes related to growth and skin mucus immunity in common carp (Cyprinus carpio)

In this study the effect of non alkohalic beer concentrate was investigated on the expression of growth hormone (GH), insulin growth factor1 (IGF1) genes and skin mucus immunity (Ig and ALP) in common carp. 180 pieces of common carp with average weight of 12.5 ± 2 g, were fed experimental diets supplemented with different levels of non alkohalic beer concentrate (0 [control], 0.5, 1 and 2%) (4 treatments and 3 replicates) for 8 weeks. At the end of feeding trial, RNA was extracted from brain and liver and cDNA synthesized using Suprime Script RTase and expression of growth related genes, GH (brain) and IGF-1(liver), were assessed using Real-time PCR. Mucosal immune indices (ALP and Ig) were measured using kit. The study of growth related genes in brain indicated significant increase of GH gene in fish fed non alkohalic beer concentrate compared to control (P<0.05). However, the results of IGF1 gene expression revealed no significant difference among treatments (P>0.05). The skin mucus ALP activity and Ig level in fish fed non alkohalic beer concentrate were significantly higher than those in control group (P<0.05). According to these results, administration of non alkohalic beer concentrate can improve immunity and growth in common carp.